Applied Virology

Modules
3 crédits ECTS
English

A propos du cours

Informations générales

This module deals with the use of embryonated chicken eggs and cell cultures for viral identification. It is offered in a blended format: web-based (distance learning) training and 1 week practical bench training at the University of Pretoria.

Objectifs d'apprentissage

Know the facilities and laboratory equipment needed to use embryonated chicken eggs and cell cultures for viral identification.
Identify the use and application of the different routes of infection of embryonated chicken eggs for viral identification.
Determine the possible signs of a viral infection in infected embryonated eggs.
Harvest appropriate material and the relevant tests used for viral identification.
Understand the different media and buffers used in cell culture systems and explain their preparation.
Perform the preparation of cell culture media and buffers used in a cell culture laboratory.
Perform the procedures to establish primary cell cultures using different starting materials.
Understand the terminology and methods used for sub-culturing different cell lines in a competent, efficient and safe manner.
Identify and detect the possible contaminants of cell cultures.
Perform and describe the procedure used for the counting and storage of cell cultures and isolated viruses.
Perform and describe the methods used to process different samples for virus isolation.
Recognize, demonstrate and discuss the presence of viruses in cell cultures.
Understand the tests used and the interpretation of results to identify viruses obtained from cell cultures and harvested infected embryonated egg material.
Perform, discuss and interpret electron microscopy, serum-viral neutralization, haemagglutination and haemagglutination inhibition tests.